Trifolium pratense
Marker Information
| Marker name | RCS3730 | ||
|---|---|---|---|
| Marker category | Red_clover_SSR | ||
| Primer sequences | Fw | GGCAAAGACCAATTTTCCAA | |
| Rv | AGAACCAACAACAACAGCCC | ||
| EST/Genome sequences | BB902943 | ||
| Lines | |||
| PIC | Value | 0.71 | |
| Lines | 48 | ||
| Linkage Map | HR x R130 | Linkage | LG4 |
| Position | 54.979 | ||
| HR x R130 | Linkage | LG5 | |
| Position | 40.890 | ||
| SNP | Position | ||
| Method | |||
| SSR | Fragment size | 196 | |
| Pattern* | AGC | ||
| Repeat count | 21 | ||
| Method | PCR | TD | |
| Detection | 10% acrylamide | ||
| Multiplex | |||
| Gel image | http://clovergarden.jp/Red/cgi-bin/photo.cgi?name=RCS3730 | ||
| Enzyme | |||
| Related markers | Marker name | AP010178, mth2-135i19 | |
| Species | Lotus japonicus, Medicago truncatula | ||
| Comments | |||
* "mis1" and "mis2" in parenthesis indicate imperfect SSR motifs having 1- and 2-base mismatches in the SSR regions, respectively. Perfect SSR motifs have no descriptions.