| Marker name | FBES0132 | ||
|---|---|---|---|
| Marker category | EST-SSR | ||
| Primer sequences | Fw | TTCAAGACATTGTTTGGGTTTG | |
| Rv | AAAGGGACTGGTACTTGTTTGTT | ||
| EST/Genome sequences | VfEES09A08 | ||
| Lines | Komasakae | ||
| PIC | Value | 0.23 | |
| Lines | 10 | ||
| SNP | Position | ||
| Method | |||
| SSR | Fragment size | 277 | |
| Pattern* | AGC(mis1) | ||
| Repeat count | 7 | ||
| Method | PCR | Modified Touchdown [Sato et al. DNA Research 12, 301–364 (205)] | |
| Detection | |||
| Multiplex | |||
| Gel image |
FBES0129-0256.ppt [download] |
||
| Enzyme | |||
| Related markers | Marker name | ||
| Species | |||
| Comments | |||
* "mis1" and "mis2" in parenthesis indicate imperfect SSR motifs having 1- and 2-base mismatches in the SSR regions, respectively. Perfect SSR motifs have no descriptions.