Marker name | FBES0776 | ||
---|---|---|---|
Marker category | EST-SSR | ||
Primer sequences | Fw | CACCAAAATTCAAAGTTATCCCA | |
Rv | ACCAGAAGGAACCCGAAACT | ||
EST/Genome sequences | VfEES10I22 | ||
Lines | Komasakae | ||
PIC | Value | 0.28 | |
Lines | 10 | ||
SNP | Position | ||
Method | |||
SSR | Fragment size | 264 | |
Pattern* | AAAT(mis2) | ||
Repeat count | 4 | ||
Method | PCR | Modified Touchdown [Sato et al. DNA Research 12, 301–364 (205)] | |
Detection | |||
Multiplex | |||
Gel image |
FBES0769-0896.ppt [download] |
||
Enzyme | |||
Related markers | Marker name | ||
Species | |||
Comments |
* "mis1" and "mis2" in parenthesis indicate imperfect SSR motifs having 1- and 2-base mismatches in the SSR regions, respectively. Perfect SSR motifs have no descriptions.